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C Laezza, IEOS, CNR, Napoli, 80131, Italy
S Pisanti, Scienze Farmaceutiche, Universita  di Salerno, Salerno, Italy
A Malfitano, Scienze Farmaceutiche, Universita  di Salerno, Salerno, Italy
M Bifulco, Scienze Farmaceutiche, Universita  di Salerno, Salerno, Italy

Correspondence: Chiara Laezza, Email: chilaez{at}hotmail.com

Abstract

The endocannabinoid system regulates cell proliferation and migration in human breast cancer cells. In this study we showed that a metabolically stable analogue of anandamide, Met-F-AEA, inhibited the RhoA activity and caused a RhoA delocalization from the cell membrane to cytosol determining a decrease in actin stress fibers. Overexpression of a dominant negative of RhoA activity and treatment of these cells with a Rho-associated protein kinase (ROCK) inhibitor, Y 27632, mimicked Met-F-AEA effects on actin organization and cell migration. We suggest that the inhibitory effect of Met-F AEA on tumour cell migration could be related to RhoA-ROCK dependent signaling pathway.