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¹ Department of Endocrinology, St Bartholomew’s Hospital, London EC1A 7BE, UK
² Department of Pathophysiology, University of Athens, Athens, Greece
³ Department of Endocrinology, G Gennimatas General Hospital, Athens, Greece

(Requests for offprints should be addressed to A B Grossman; Email: a.b.grossman{at}qmul.ac.uk)

	Abstract

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
Neuroendocrine tumours (NETs) constitute a heterogeneous group of tumours that frequently express cell membrane-specific peptide receptors, such as somatostatin receptors (SSTRs), and of which gastroenteropancreatic (GEP), carcinoid and pancreatic islet cell tumours exhibit the highest expression of SSTRs. Radiolabelled receptor-binding somatostatin analogues (octreotide and lanreotide) act as vehicles to guide radioactivity to tissues expressing SSTRs, and can thus be used for their diagnosis and treatment. After the localization of NETs bearing SSTRs with ¹¹¹In-octreotide (OctreoScan), a number of radioisotopes with different physical properties have been used for their treatment. The administration of high doses of the Auger electron and -emitter ¹¹¹In-diethylenetriaminepenta-acetic acid (DTPA)⁰,octreotide in patients with metastatic tumours has been associated with considerable symptomatic improvement but relatively few and short-lived objective tumour responses. The use of another radiolabelled somatostatin analogue coupled with ⁹⁰Y, a pure ß-emitter, ⁹⁰Y-1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA)⁰,Tyr³,octreotide (⁹⁰Y-DOTATOC, OctreoTher), was associated with 10–30% objective tumour response rates, and appears to be particularly effective in larger tumours. ¹¹¹In- and ⁹⁰Y-DOTA-lanreotide has also been used for the treatment of NETs although its therapeutic efficacy is probably inferior to that of octreotide-based radiopharmaceuticals. More recently, treatment with ¹⁷⁷Lu-DOTA⁰,Tyr³octreotate (¹⁷⁷Lu-DOTATATE), which has a higher affinity for the SSTR subtype 2, resulted in approximately 30% complete or partial tumour responses; this radiopharmaceutical is particularly effective in smaller tumours. Furthermore, treatment using both ⁹⁰Y-DOTATOC and ¹⁷⁷Lu-DOTA⁰,Tyr³octreotate seems promising, as the combination of these radiopharmaceuticals could be effective in tumours bearing both small and large lesions. Tumour regression is positively correlated with a high level of uptake on ¹¹¹In-octreotide scintigraphy, limited tumour mass and good performance status. In general, better responses have been obtained in GEP tumours than other NETs. The side effects of this form of therapy are relatively few and mild, particularly when kidney-protective agents are used. Treatment with radiolabelled somatostatin analogues presents a promising tool for the management of patients with inoperable or disseminated NETs, and particularly GEP tumours.

	Introduction

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
Neuroendocrine tumours (NETs) constitute a heterogeneous group of tumours that originate from the diffuse endocrine system (DES). This system includes endocrine glands, such as the pituitary, the parathyroids and the adrenal medulla, as well as endocrine islets within glandular tissue (thyroid or pancreatic) and cells disseminated between exocrine cells, such as endocrine cells of the digestive and respiratory tracts (Arnold et al. 1994, Kaltsas et al. 2004a). The latter are usually known as ‘carcinoid tumours’, and, according to their anatomic site of origin, they have been classified as foregut, midgut and hindgut carcinoid tumours (Solcia et al. 1999). Within these subgroups, the biological behaviour of the tumours can vary significantly (Oberg 2004a, Oberg et al. 2004). NETs occur either sporadically or as part of familial syndromes, and although some may show aggressive behaviour and become highly malignant (poorly differentiated NETs), the great majority are either benign or relatively slow growing (well-differentiated NETs), retaining many multipotent differentiation features (Kaltsas et al. 2001a). Such features include the possession of neuroamine uptake mechanisms and/or specific receptors at the cell membrane, such as somatostatin receptors (SSTRs), which can be used for the identification, localization and therapy of NETs (Oberg 2002, 2004a). Of the five major subtypes of SSTR, SSTR_1–5, SSTR₂ and SSTR₅ are the ones most commonly expressed in NETs; however, there is considerable variation in SSTR subtype expression among the different tumour types and among tumours of the same type (de Herder et al. 2003).

Radiopharmaceuticals are radiolabelled substances that can be utilized for the identification and localization of NETs by their ability to bind to suitable ligands (Kaltsas et al. 2001b). They form an imaging modality which is based on the physiological (the presence of functioning receptors) rather than the anatomical characteristics of the tumours (de Herder et al. 1996). As the majority of NETs express SSTRs they form an ideal target for treatment with somatostatin analogue (SST analogue)-derived radiopeptides (Krenning et al. 1999a, de Herder et al. 2003). SST analogues are synthetic peptides (octreotide, Novartis, Basel, Switzerland, and lanreotide, Ipsen, Paris, France) that exert most of the biological actions of the native peptide somatostatin, but have a longer half-life (2 min vs ~ 90 min), being resistant to plasma degradation (de Herder et al. 2003). The high affinity of these peptides for SSTRs (highest affinity for SSTR₂, moderately high for SSTR₅ and intermediate for SSTR₃) and the internalization of the receptor–peptide complex facilitate retention of the radiopeptide in receptor-expressing tumours, whereas their relatively small size facilitates rapid clearance from the blood (Reubi et al. 2004). In order to ensure the stability of the molecule, the peptide labelled with a radioisotope is bound to a chelator. Covalently linking ethylene-triamine-pentaacetic acid (DTPA) with octreotide results in DTPA-octreotide (pentetreotide); this tracer was initially labelled with ¹²³I, but it gradually became evident that it is more clinically effective in localizing NETs when labelled with ¹¹¹In (Kaltsas et al. 2004a) (Fig. 1). Subsequently, labelled lanreotide was also introduced, but the images obtained with this radionuclide were generally inferior to those of octreotide (Virgolini et al. 2002a,b).

View larger version (21K): [in this window] [in a new window]   Figure 1 Currently used ligands for treatment with radiopeptides based on somatostatin analogues.

After the introduction of radiolabelled octreotide for the diagnosis of lesions expressing SSTRs, the next step was to use this radiopharmaceutical to develop specific therapy, particularly for inoperable or multiple-site tumours showing diagnostic uptake of ¹¹¹In-octreotide (Lewington 2003). As SSTRs are mainly expressed in GEP tumours, these tumours represent the main target group for therapy with radiolabelled peptides (de Herder et al. 2003).

	Basic concepts of applying therapy with radiopharmaceuticals to NETs

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
The initial management of NETs is surgical excision of the primary tumour, unless specific medical therapy is available. This form of treatment usually suffices for the majority of benign tumours, but recurrent, malignant and disseminated tumours require further treatment; this is particularly relevant to the majority of GEP tumours (Oberg et al. 2004). SST analogues and interferon-alpha (IFN-) can achieve disease stabilization but, eventually, further treatment will be required for primary and metastatic tumours that continue to grow; this has traditionally been in the form of systemic chemotherapy and external radiotherapy for local control (Kaltsas et al. 2004b, Oberg et al. 2004). Although systemic chemotherapy can be temporarily effective in the minority of patients with poorly differentiated NETs, the majority of well-differentiated NETs are relatively chemoresistant (Kaltsas et al. 2004b, Oberg et al. 2004). Chemotherapy itself is also associated with considerable side effects, and thus its use must be weighed against potential adverse effects (Kaltsas et al. 2004b). Different forms of treatment with a more favourable side-effect profile are highly required for patients with well-differentiated metastatic NETs, particularly as such patients can experience prolonged survival in the presence of extensive disease (Oberg 2004b, Oberg et al. 2004).

The application of therapy with radiopharmaceuticals to NETs is dependent on the concept that coupling a radioisotope to a molecule which would specifically bind to tumour cells could deliver an effective radiation dose to the tumour without damaging healthy tissues, thus limiting adverse effects (Krenning et al. 1999a). The success of this approach depends upon the amount of radioligand that can be concentrated within tumour cells and the rates of internalization, degradation and recycling of both ligand and receptor (Wiseman & Kvols 1995). The radioisotope’s physical properties are important for targeting radiation therapy because even though tumour heterogeneity can cause incomplete responses, crossfire from the radioisotope localized on target-positive tumour cells can kill the nearby tumour cells that are target negative (Krenning et al. 2004, Kwekkeboom et al. 2005, Pauwels et al. 2005, Reubi 2005). The radioisotope selection is based on the type of radiation emitted, the emission energies, the distance over which the energy is deposited, and the physical half-life of the radioisotope (Tables 1 and 2). According to this concept, ß-, - or Auger electron emitters can deliver a cytotoxic dose of radiation with a high linear energy transfer selectively to tumours expressing SSTRs; the ß-emitter selected must have an appropriate path to reach the cell nucleus (probably the principal, although not the sole, target to achieve cell death) depending upon the site of cellular radiopharmaceutical concentration (Siegel & Stabin 1994, Lewington 2003). Physical half-life directly relates to the rate at which the absorbed radiation dose is delivered; a higher dose rate is best suited to rapidly dividing tumours, whereas a longer physical half-life may be more effective for relatively slow-growing tumours such as the majority of NETs (Lewington 2003, Pauwels et al. 2005).

Targeted radiotherapy for NETs (mainly GEP tumours) is mostly performed with radiopeptides based on SST analogues; the ligands that are currently in clinical use are shown in Fig. 1 (DTPA octreotide, DOTA-_D Phe-tyr³-octreotide (DOTA TOC), DOTA-_D Phe-Tyr³-octreotate (DOTATATE) and DOTA-lanreotide) (Krenning et al. 2005, Reubi et al. 2005). ¹¹¹In-DTPA exhibits moderate binding affinity for SST₂ (negligible for SST_1,3–5), while ¹¹¹In is mainly an Auger electron emitter exhibiting only short-range radiotoxicity. However, DTPA is not an ideal chelator for the currently used ß-particle emitters such as yttrium-90 (⁹⁰Y) and lutetium-177 (¹⁷⁷Lu); for these radioisotopes, the macrocyclic chelator 1,4,7,10-tetra-azacyclododecane-N,N',N'',N'''-tetraacetic acid (DOTA) is better, as it forms stable complexes with the isotopes. The DOTA-coupled, somatostatin-based radiopharmaceuticals are ⁹⁰Y-DOTA⁰-Tyr³-octreotide (⁹⁰Y-DOTATOC), ⁹⁰Y-DOTA-lanreotide and ¹⁷⁷Lu-DOTA-Tyr³-Thre⁸-octreotide (¹⁷⁷Lu-DOTA-TATE), shown in Fig. 2. These differ with regard to SST receptor affinity profile (Table 3), the DOTA-TATE derivative exhibiting the highest affinity to SSTR₂ and DOTA-lanreotide having the lowest affinity to SSTR₂ although demonstrating considerable SSTR₅ affinity (Krenning et al. 2005). The various factors that may determine the amount of uptake of radiolabelled SST analogues have been recently outlined as follows:

View larger version (20K): [in this window] [in a new window]   Figure 2 Chemical structure of currently used octreotide-based radiopharmaceuticals for the treatment of gastroenteropancreatic and other neuroendocrine tumours (OctreotherTM = 90Y-SMT487 = 90Y-DOTATOC).

1. the stability of the radioligand
   
2. the density of SSTR expression on the tumour
   
3. the type of SSTRs expressed by the tumour
   
4. the affinity of the radioligand for the SSTRs
   
5. the efficiency of SSTR-mediated internalization and recycling
   
6. the final trapping of the radioisotopes within the tumour cells
   
7. the mass of the injected peptide (de Herder et al. 2003).
   

	Dosimetry

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
Radiopharmaceuticals are taken up by both tumour cells and normal tissues, and this form of therapy aims to deliver the highest possible dose to the tumour while sparing normal tissues from damage (Pauwels et al. 2005). As in other forms of treatment aiming at the elimination of neoplastic cells, critical dose-limiting organs in therapy with radiopharmaceuticals are the bone marrow and the kidneys, the latter due to interstitial irradiation derived from tubular peptide reabsorption (Bodei et al. 2004a). This was evident from preliminary clinical trials using ¹¹¹In- and ⁹⁰Y-labelled peptides in which the considerable therapeutic benefits noted in patients with progressive disease were associated with significant, mostly delayed toxicity leading to substantial morbidity (Otte et al. 2002, Valkema et al. 2002). Dosimetry methods can be used to provide reliable dose estimates to critical organs and to tumoral tissues before therapy, leading to individualized therapeutic regimes; dosimetry can help to identify patients who would benefit from therapy with radiopharmaceuticals while excluding unsuitable patients (Bodei et al. 2004a, Pauwels et al. 2005). These estimations require time-consuming and sophisticated methods, including pharmacokinetic, biodistribution and wash-out studies involving the selected radiopharmaceutical to be used for therapy (Kassis & Adelstein 2005, Pauwels et al. 2005).

Selection of the radiopharmaceutical

The chemical structure of the radiopharmaceutical is important, as it influences tumour uptake, renal clearance and retention, and residence time in tissues (Reubi et al. 2000). Dosimetry with ¹¹¹In-octreotide can easily be performed when this same radiopharmaceutical is to be used for treatment (Cremonesi et al. 1999, Pauwels et al. 2005). However, this is not possible with ⁹⁰Y-radiopharmaceuticals, as the ß-emission of ⁹⁰Y does not allow appropriate quantification from the -activity emitted by affected cells, necessitating the use of alternative approaches. Imaging with the peptide surrogate labelled with the positron emitter ⁸⁶Y was precise in measuring distribution and retention of the radiopharmaceutical over time, but a major drawback is the requirement of a positron emission tomography (PET) facility and a high-energy cyclotron (Pauwels et al. 2005). A different method to overcome the limitations of using ⁸⁶Y was based on the evaluation of the biodistribution and dosimetry of DOTATOC radiolabelled with ¹¹¹In (Cremonesi et al. 1999); however, biodistribution studies using ¹¹¹In-DTPA octreotide have demonstrated either under- or overestimation of renal doses of ⁹⁰Y-DOTATOC (Pauwels et al. 2005). Additional improvements in the determination of the kidney radiation dose can be obtained with the precise organ volume measurement (instead of a fixed volume) by computerized tomography (CT) or magnetic resonance imaging (MRI) (Barone et al. 2005). Patient-specific dosimetry requires quantitative imaging of the patient at different times points (according to the half-life of the radioisotope used) to evaluate the retention of activity in various organs over time. After integration of the measured activities, time integrals of activity are multiplied by energy-transport factors to obtain radiation dose estimates in target organs. Once these activities are measured, absorbed doses in target organs are calculated with dedicated software (Sgouros 2005). In cases where the absorbed dose is mainly related to self-irradiation, the energy transported per decay from the source tissue to the mass of the target tissue (S factor) depends on the target volume; improvements in the accuracy of measured doses may thus be achieved by organ volume estimation using CT or MR imaging (Pauwels et al. 2005, Sgouros 2005).

Although it is clear that accurate prediction of absorbed doses is required for internal radiotherapy, inaccuracies can occur even with the use of highly sophisticated techniques, leading to miscalculation of the absorbed doses: this will lead to either under- or overtreatment with unpredictable toxicity (Pauwels et al. 2005).

Evaluation of response

Patients’ responses to therapy should be evaluated by uniform criteria and divided into symptomatic, hormonal and tumour responses (Kaltsas et al. 2004a). Tumour responses are classified by WHO criteria as follows:

1. complete response, complete regression of all clinical and hormonal evidence of tumour, including radiological abnormalities
   
2. partial response, a 50% or greater reduction of all measurable tumour and no appearance of new lesions, along with hormonal and symptomatic improvement
   
3. stable disease, a less than 50% reduction or no greater than 25% increase of tumour size, hormonal measurements and symptoms
   
4. progression, appearance of new lesions or an increase of 25% or more of tumour size, and hormonal and symptomatic deterioration.
   

Patients are reviewed at 3–6-month intervals; review assessment comprises clinical, biochemical and radiological evaluation. The rationale of applying further treatment at these time intervals is contingent on the relatively prolonged replication period of these tumours (Kaltsas et al. 2004b).

	Pretreatment considerations

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
A large number of NETs, mainly GEP tumours (functioning or non-functioning), are being treated with SST analogues; this is particularly important, as many of these tumours are associated with severe humoral syndromes (functioning tumours) for the control of which treatment with SST analogues is continuously required (Kaltsas et al. 2004b, Oberg 2004b). During treatment with these drugs, the uptake of ¹¹¹In-DTPA-octreotide in SST-positive tumours and the spleen is diminished (similar to blocking the receptor binding of a labelled ‘hot’ peptide with an excessive amount of unlabelled ‘cold’ peptide). In practice, NETs may still remain visible during treatment with octreotide, although tumour uptake of ¹¹¹In-octreotide may be substantially reduced. In order to maximize the uptake of radiolabelled compounds, patients on long-acting releasable (LAR) SST analogues should receive treatment with radiopharmaceuticals before the next LAR administration (4–6 weeks), or after they have been switched to s.c. octreotide, which has a much shorter half-life and can be discontinued 24 h before therapy (Kwekkeboom et al. 2005). Particular attention should be paid to patients with extensive hepatic involvement and high uptake on the diagnostic OctreoScan (Mallinckrodt Pelten, The Netherlands). These patients are at high risk of developing fulminant hepatic necrosis, as extremely high radiation may be delivered to the small number of normal liver cells from the increased uptake of the neighbouring metastatic foci (Kwekkeboom et al. 2005). In such cases, lowering the cycle dose is an alternative if treatment with radiopharmaceuticals is required (see below). In addition, when there is increased uptake in the bone marrow in patients with disseminated disease, it is important to exclude metastatic infiltration of the bone marrow, as treatment with radiopharmaceuticals may exacerbate the bone marrow failure after therapeutic radiation (Mukherjee et al. 2001). This is also important for patients who have previously undergone intensive chemotherapy (Bodei et al. 2004b).

Peptide receptor radionuclide therapy with ¹¹¹In-octreotide

¹¹¹In emits mainly -radiation, which passes through tissue relatively easily and can be imaged by a -scanner. ¹¹¹In also emits Auger and conversion electrons that exert an anti-proliferative effect in malignant tumours if their major target, the DNA of the cell, is within the particle range (de Jong et al. 2002). In a study by Krenning et al. (1999b), 30 end-stage patients with NETs, including 20 patients with GEP tumours, were treated with [¹¹¹In-DTPA]-octreotide, receiving a cumulative dose of 20 GBq (550 mCi). Seven patients who were treated with a total dose of less than 20 GBq ¹¹¹In-DTPA-octreotide had to stop therapy prematurely due to the extension of their disease despite treatment. Of the 21 patients who received adequate doses of ¹¹¹In-DTPA-octreotide (all had progressive disease), six demonstrated partial shrinkage of the tumour, while eight others showed stabilization of their disease; patients with higher tumour uptake tended to show better results (Krenning et al. 1999b). Similar results were obtained in a study that included 40 patients who received cumulative doses of 20–160 GBq. Therapeutic effects were seen in 21 patients: partial remission in one, minor remissions in six and stabilization of previously progressive tumours in 14 (Valkema et al. 2002). Nevertheless, three of the six patients who received more than 100 GBq (2.7 Ci) developed a myelodysplastic syndrome or leukaemia, and the authors concluded that 100 GBq are the maximal tolerable dose. In another study, 27 patients were treated with two doses of 6.5 GBq (180 mCi) ¹¹¹In-pentetreotide: objective partial responses occurred in two (8%) patients and significant tumour necrosis in seven (27%) (Anthony et al. 2002). Symptomatic and hormonal response was obtained in 62% and 81% respectively, whereas median survival was 18 months compared with the 6 months expected, without any major side effects (Anthony et al. 2002). However, a more recent study has challenged the long-term efficacy of ¹¹¹In-pentetreotide therapy in patients with disseminated NETs (Buscombe et al. 2003). This is not surprising, as ¹¹¹In-coupled peptides are not ideal for therapy with radiopharmaceuticals because of the small particle range and therefore lower tissue penetration rate; short-lived responses have been attributed to the preferential survival of resistant clones (de Jong et al. 1997, 2003, Kwekkeboom et al. 2005). Moreover, its efficacy in large tumours and end-stage patients is limited, reflecting mainly heterogeneous radiopharmaceutical uptake due to poor tumour vascularity and central necrosis (Lewington 2003). It has been therefore been suggested that ¹¹¹In-octreotide should be given only with high-energy ß-particle emitters in order to eradicate micrometastasis (de Jong et al. 2002), particularly as various studies with ß-emitters alone have demonstrated better response rates (Valkema et al. 2002, de Jong et al. 2005, Kwekkeboom et al. 2005) (see below).

	Peptide receptor radionuclide therapy with (90Y-DOTA 0Tyr3)-octreotide [90Y- DOTATOC]

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
Radiopeptides emitting ß-radiation have superior therapeutic potential since the particles they emit have sufficient energy to cause cell damage without penetrating very far into surrounding tissue (Wiseman & Kvols 1995). A high-energy ß-emitter attached to a SST analogue could potentially deliver a lethal dose of radiation to a SST-positive tumour with minimal damage to healthy tissue (Wiseman & Kvols 1995, de Jong et al. 2003). Despite its diagnostic use, DTPA-octreotide is not suitable for labelling with yttrium-90 (⁹⁰Y), a pure energetic ß-emitter well suited for internal radiotherapy; this has led to the development of a new peptide chelator that can be stably labelled with ¹¹¹In and ⁹⁰Y (de Jong et al. 1997, Krenning et al. 1999a). The same SST analogue (Tyr³-octreotide) used for diagnosis has been coupled to the bifunctional chelating agent (1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid, DOTA⁰) for the complexing of ⁹⁰Y (de Jong et al. 1997, Krenning et al. 1999a) (Fig. 2). In contrast to ⁹⁰Y-DTPA-octreotide, ⁹⁰Y-DOTA-D-Phe¹-Tyr³-octreotide (⁹⁰Y-SMT487, OctreoTher, ⁹⁰Y-DOTATOC) shows no dissociation from the DOTA-chelated peptide in serum and can deliver tumouricidal doses of radiation by targeting SST-positive tumours that have demonstrated uptake on a diagnostic ¹¹¹In-pentetreotide scan (OctreoScan) (de Jong et al. 1997). Furthermore, while, in general, ⁹⁰Y-DOTA-D-Phe¹-Tyr³-octreotide is a non--emitter, the ß-particles can cause localized release of -activity from affected cells (Bremsstrahlung), making some imaging of the effectiveness of therapy possible (Kaltsas et al. 2004a) (Fig. 3).

View larger version (101K): [in this window] [in a new window]   Figure 3 Reconstructed SPECT image of a malignant insulinoma imaged with 90Y-octreotide at 24 h. Note that the primary tumour is clearly shown (Indicated by the cross). The radiation from this predominantly ß-emitter is secondary radiation, Bremsstrahlung.

Despite differences between the several protocols used, most of the studies using ⁹⁰Y-DOTATATE have demonstrated tumour responses of 10–30% (mean 25%), considerably higher than those obtained with ¹¹¹In-DTPA (Table 4). These differences could be attributed either to differences in administered doses and dosage schemes between the various studies or, more likely, to differences in patient characteristics (percentage of radionuclide uptake, estimated total tumour burden and extent of liver involvement). In addition, several studies have included different types of NETs with considerable differences in the density of SSTRs and natural history. As already mentioned, when response is considered according to the various types of NETs, it becomes evident that patients with GEP tumours exhibit a more favourable response, 36–38%, than other NETs treated with the same radiopharmaceuticals (Waldherr et al. 2001, Bodei et al. 2004a, Kwekkeboom et al. 2005) (Table 4).

	Peptide receptor radionuclide therapy with 90Y-lanreotide

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

A few patients with malignant chromaffin cell tumours have been treated with ¹¹¹In-DTPA-octreotide and ⁹⁰Y-DOTATOC, some obtaining disease stabilization (Waldherr et al. 2001, Valkema et al. 2002). A few patients with medullary carcinoma of the thyroid (MTC) also received ¹¹¹In-octreotide or ⁹⁰Y-lanreotide in the MAURITIUS trial (Krenning et al. 1999a, Virgolini et al. 2002b, Buscombe et al. 2003). Although this was a heterogeneous group of patients, initial results were encouraging, supporting further prospective studies of the use of this therapy in patients with advanced MTC (Krenning et al. 1999a, Virgolini et al. 2002b, Buscombe et al. 2003).

	Peptide receptor radionuclide therapy with [177Lu-DOTA-Tyr3]octreotate

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
In order to overcome the limitations of administering tumoricidal doses of radiotherapy to non-octreotide-avid lesions, and the lack of uptake to certain areas due to tumour heterogeneity, several ß-emitters, in addition to ⁹⁰Y, such as ¹⁵³Sm, ¹⁷⁷Lu and ¹⁸⁶Re, have also been considered, usually in combination with Tyr³-octreotate (DOTATE), which exhibits the highest affinity for SSTR₂ (de Jong et al. 2002). Initial studies reported a ninefold increase in the affinity for SST₂ of DOTA Tyr³-octreotate compared with DOTA-Tyr³-octreotide, which remained six- to seven-fold higher when ⁹⁰Y was also added to the complex (Reubi et al. 2005). Besides the higher tumour uptake, Tyr³-octreotate exerts similar kidney uptake to Tyr³-octreotide, leading to a better tumour : kidney ratio; however, Tyr³-octreotide exhibits faster renal clearance, and this could in theory mean lower bone-marrow irradiation (de Jong et al. 2002). The relative advantages of ¹⁷⁷Lu over ⁹⁰Y include also the emission of -radiation, which makes it available for scintigraphy/dosimetry, and the shorter range of ß-particles, by which higher radiation can be delivered to smaller tumours (de Jong et al. 2003). Therefore, ¹⁷⁷Lu-DOTA-Tyr³-octreotate may well represent a radiopharmaceutical advance, as it can achieve higher absorbed doses for most tumours with similar doses to dose-limiting organs because of the lower tissue-penetration range than ⁹⁰Y-DOTATOC, a feature particularly important for smaller lesions (de Jong et al. 2003).

An initial study assessed the effects of ¹⁷⁷Lu-DOTA-Tyr³-octreotate therapy on tumours of various sizes in 34 patients with GEP tumours (Kwekkeboom et al. 2003). Three months after the final administration, complete remission was found in one patient (3%), partial remission in 12 (35%) patients, stable disease in 14 (41%) patients and progressive disease in seven (21%) patients. The outcome of radionuclide therapy was positively correlated with a high uptake on the OctreoScan, limited hepatic tumour mass and high performance status (Kwekkeboom et al. 2003). The authors also noted that responding patients had a limited tumour load, thus indicating early employment of receptor radionuclide therapy in patients with GEP tumours, without waiting for tumour progression (Kwekkeboom et al. 2003). The same group have recently assessed the effect of ¹⁷⁷Lu-DOTA-Tyr³-octreotate on the self-reported QoL in patients with metastatic GEP tumours (Teunissen et al. 2004). The global health/QoL, as well as several function and symptom scales, was significantly improved after ¹⁷⁷Lu-DOTA-Tyr³-octreotate therapy even in patients with progressive disease (Teunissen et al. 2004). More recently, the results of 131 patients with GEP tumours treated with a cumulative dose of 22.2–29.6 GBq of ¹⁷⁷Lu-DOTA-Tyr³-octreotate became available: three patients (2%) obtained complete remission; 32 (26%), partial remission; 24 (19%), a minor response (tumour diameter decrease of 25–50%); and 44 (35%), stable disease. Twenty-two (18%) patients developed tumour progression. Serious side effects were rare, only a single patient developing acute renal failure and one developing hepatorenal syndrome, whereas the median time to progression for patients who either responded or remained stable was 36 months (Kwekkeboom et al. 2005). Patients who obtained lower remission rates and/or progression of their disease had lower uptake on the diagnostic ¹¹¹In-octreotide scan, more extensive disease and hepatic involvement, and worse performance status. These results compare favourably to the ones obtained in patients treated with chemotherapy, and imply that treatment efficacy is probably related to early initiation of treatment when tumour load is limited (Kwekkeboom et al. 2005). A further finding of the latest study favours the early administration of treatment with radiopharmaceuticals, as disease stability or progression before the initiation of treatment did not affect the response to treatment (Kwekkeboom et al. 2003).

From these studies, where several different radio-pharmaceuticals have been used for the treatment of NETs, it seems that ¹⁷⁷Lu-DOTA-Tyr³-octreotate represents a major advance in the field of treatment with radiopeptides. However, it must be emphasized that direct, randomized, comparative studies of the various forms of radiopharmaceuticals used are lacking.

	Combination treatment with 90Y-DOTATOC and 177Lu-DOTA-Tyr3-octreotate

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
When we consider the individual radiobiological features of the ß-emitters ¹⁷⁷Lu and ⁹⁰Y, it becomes evident that each has specific potential advantages for tumour therapy (Tables 1 & 2). ⁹⁰Y particles have higher energies and longer ranges, leading to the deposition of more radioactivity in tumour cell per peptide molecule and to a better crossfire throughout the tumour; in addition, the relatively short half-life of ⁹⁰Y leads to a higher dose rate. These properties make it more favourable for the treatment of larger tumours and for tumours with heterogeneous receptor distribution (de Jong et al. 2005). However, smaller tumours are less suitable for treatment with ⁹⁰Y, as they will not absorb all the energy emitted from ⁹⁰Y in the tumour cells, whereas adjacent healthy tissue will absorb relatively high doses and will be exposed to toxicity (Krenning et al. 2004). In contrast, ¹⁷⁷Lu particles that have lower energy and a smaller particle range are associated with better absorption in smaller tumours, and enable scintigraphy, as they emit -radiation (de Jong et al. 2005). Therefore, to treat patients with tumours of various sizes and non-homogeneous receptor distribution, the combination of the high-energy ⁹⁰Y for the larger lesions and the low-energy ¹⁷⁷Lu for the smaller lesions and metastases seems a reasonable approach. Animal studies have documented that this combination (⁹⁰Y and ¹⁷⁷Lu) provides different ß-energies and particle ranges that achieve higher cure rates in tumours of various sizes than either radionuclide alone (de Jong et al. 2005). These results also agree with the prediction of a mathematical model that was used to assess tumoral response to different ß-emitting particles in relation to tumour size, and that suggested an optimal tumour diameter of 34 mm for ⁹⁰Y and 2 mm for ¹⁷⁷Lu (O’Donoghue et al. 1995). Another interesting option is the sequential treatment of these tumours with these analogues, initially administering ⁹⁰Y-labelled analogues to treat larger lesions followed by ¹⁷⁷Lu-labelled analogues to treat smaller metastases (de Jong et al. 2005).

	Predictors of response

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Despite variations of the protocols used, objective tumour size responses of approximately 25% (range 10–30%) have been obtained in studies using ⁹⁰Y-DOTATOC (Chinol et al. 2002, Waldherr et al. 2002, Bodei et al. 2003, Valkema et al. 2005), and approximately 30% in those using ¹⁷⁷Lu-¹⁷⁷Lu-DOTA-Tyr³-octreotate (DOTATATE) (Krenning et al. 2005, Kwekkeboom et al. 2005, Pauwels et al. 2005). This rather low response rate may be related to the relative radioresistance of NETs, but probably also reflects the absence of uniform criteria in patient selection (Bodei et al. 2004b, Pauwels et al. 2005). It is therefore important to establish predictive parameters to select patients likely to benefit from treatment with these radiopeptides and identify those not likely to respond. As shown from data derived from patients with NETs treated with ¹³¹I-MIBG, important predictors of the outcome of therapy with this radionuclide are the extent of the disease and the total tumour load (Chatal et al. 2000, Mukherjee et al. 2001). Similarly, tumour regression was positively correlated with high level of uptake on OctreoScan imaging, limited hepatic tumour mass and high Karnofsky performance score (Kwekkeboom et al. 2003). There is also evidence of a significant correlation between dose estimates of tumours and tumour size reduction that is measured at the end of the treatment; the median absorbed dose was sixfold higher in responding than non-responding tumours (Pauwels et al. 2005). Although tumour dosimetry did not completely distinguish responders from non-responders, it may be useful in the management of patients with NETs. It has therefore been suggested that early treatment (particularly in patients with progressive disease) may be associated with better outcome (Kwekkeboom et al. 2003). The fact that progression during or after treatment was more frequent in patients with extensive tumour load, particularly in the liver, implies that a more aggressive approach may be more appropriate than a ‘wait-and-see’ approach, as this might be associated with worse outcome (Kwekkeboom et al. 2003). In addition, future studies should provide evidence-based information on the optimal time to treat with radiopeptides with respect to other forms of treatment, and whether disease stability should be regarded as response to treatment, particularly in the group of patients with progressive disease following other forms of treatment (Bodei et al. 2004b). However, we now have sufficient information to suggest rational indications for the possible use of this type of treatment (Table 5).

	Radioembolization

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	Safety and side effects

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Biodistribution studies demonstrated that the highest predicted absorbed doses were to the spleen, kidneys and the tumour. Preliminary studies showed that since no spleen toxicity was demonstrated, the kidneys are the critical organs, particularly as octreotide is mainly excreted by the kidneys (Bodei et al. 2003, Pauwels et al. 2005). After being filtered by glomeruli, radio-peptides are reabsorbed and retained from the proximal tubule; renal toxicity occurs because of irradiation of the radiosensitive glomeruli by activity retained in the relatively radioresistant tubular cells, the main histological finding being thrombotic microangiopathy (Otte et al. 2002, Pauwels et al. 2005). The cumulative absorbed dose to the kidney may cause renal damage when it exceeds the conventional threshold of 23–25 Gy; although this threshold is derived from experience obtained from external irradiation, cases of several renal failure have been found to follow treatment with radiopharmaceuticals (Cybulla et al. 2001, Otte et al. 2002). Adjustments of the threshold dose by the linear-quadratic method has estimated the renal threshold toxicity dose to be 35 ± 7 Gy for ⁹⁰Y-DOTATOC (Konijnenberg 2003). Although renal uptake may vary considerably in patients undergoing dosimetry, this can be substantially improved when the actual kidney volume (as estimated by CT) is taken into consideration (Pauwels et al. 2005). Retrospective analysis of patients who have developed renal toxicity revealed that this occurred in patients who had the smaller kidneys (received higher radiation doses per ml) (Pauwels et al. 2005, Valkema et al. 2005). In addition, methods to reduce kidney uptake and thus avoid acute and/or delayed renal toxicity have been developed (Pauwels et al. 2005, Valkema et al. 2005). Positively charged amino acids, such as L-lysine and/or L-arginine, have been shown to completely inhibit proximal tubular reabsorption of the radiopeptides, thus resulting in a potential deduction in the renal dose. Different regimens of amino-acid co-infusion with a 27% mean reduction (range 20–40%) in the renal absorbed dose and no effect on tumoral uptake have been described; no further cases of renal insufficiency have been described in studies treating a considerable number of patients with NETs with this regimen (Bodei et al. 2004a, Pauwels et al. 2005, Valkema et al. 2005).

Renal toxicity was not the only parameter to be considered. Recent studies have shown that SST analogues are also taken up from the bone marrow, but not the trabecular cortical bone; although cases of bone-marrow toxicity were described in the initial studies, several subsequent studies treating a large number of patients failed to confirm these data (Bodei et al. 2004a, Pauwels et al. 2005). However, the possibility of a mild but progressive deficiency in bone-marrow reserves has to be considered, and this form of treatment should probably not be administered to patients with metastatic disease involving the bone marrow extensively (Bodei et al. 2004a). Following the development of acute hepatic toxicity in patients with extensive hepatic involvement, Bushnell et al. (2003a) studied 12 patients with extensive hepatic metastases (defined as 25% or more) who were treated with ⁹⁰Y-DOTATOC. Only four patients developed a significant elevation of hepatic enzymes, suggesting that such patients can be treated with a cumulatively administered activity of 360 mCi of ⁹⁰Y-DOTATOC with only a small chance of developing mild, acute or subacute hepatic radiation damage (Bushnell et al. 2003a). Neither endocrine dysfunction of the pituitary axis nor diabetes mellitus has been observed after treatment with radiopharmaceuticals (Bodei et al. 2004a); however, transient impairment of spermatogenesis has been described (de Jong et al. 2002).

	Future prospects

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Targeting antigens or receptors that are overexpressed in tumour cells compared with the expression in normal cells, or exploiting uptake mechanisms, has been the basis for therapy with radiopharmaceuticals. To optimize their effect, this form of treatment should be used in patients with small tumour targets (not more than 1–2 cm in diameter) (de Jong et al. 2002). For large, macroscopic targets, internal radiotherapy is probably inefficient because of the low, heterogeneous tumour uptake (Krenning et al. 2005). Combination therapy with both ¹³¹I-MIBG and ⁹⁰Y-DOTATATE or the combinations of ¹¹¹In/⁹⁰Y/¹⁷⁷Lu-octreotide remains an attractive theoretical option, after as complete a surgical resection as possible, to ensure good uptake of the radiopharmaceutical by the remaining tumour target (Krenning et al. 2005, Larson & Krenning 2005). If treatments are repeated regularly, it is likely that the disease can be at least stabilized for a period of several years, if not cured (Kaltsas et al. 2004a).

In addition, newer SST analogues in combination with different emitters are in development that might exhibit superior imaging and therapeutic properties. SOM230 (Novartis, Basel, Switzerland) is a newly developed SST analogue that binds with high affinity to SSTR_1–3 and SSTR₅, but not SSTR₄ (Bruns et al. 2002, de Herder et al. 2003). Its potential use as a radiopharmaceutical is awaited with interest. In addition, new drugs interacting with multireceptor family cross-talk, particularly SSTRs, are being developed; these SST subtype homo- or heterodimers may exert properties distinct from the individual receptors in terms of internalization and functional activity (de Herder et al. 2003). Hybrid somatostatin-dopamine molecules with high affinity to SSTR₂ and D₂ receptors have been developed and are expected to have a higher affinity than each receptor alone in NETs that express them (de Herder et al. 2003).

Apart from expressing SSTRs, GEP tumours can express several other receptors such as glucagon-like peptide 1 (GLIP-1) receptors, cholecystokinin receptors, and bombesin or vasoactive intestinal peptide (VIP) receptors (Reubi & Waser 2003). Thus, insulinomas have more GLIP-1 receptors than SSTRs whereas carcinoid tumours also express cholecystokinin 2, bombesin and/or VIP receptors (Reubi 2004). The concomitant expression of several of these peptide receptors in GEP tumours forms the basis for in vivo multireceptor targeting of those tumours, using a combination of radiolabelled compounds (de Jong et al. 2003, Reubi et al. 2005). Such an approach may increase the accumulation of radioactivity in these tumours, improving further targeting efficacy, and it may also allow different radioligands to be labelled with isotopes of different ranges in order to obtain optimal radiotherapy for lesions of various size. Radiolabelled minigastrin analogues have already been applied successfully in cholecystokinin 2 receptor-positive tumours, such as MTC (de Jong et al. 2003, Reubi 2005).

Compounds such as RGD (Arg-Gly-Asp) peptides have been described as antagonizing tumour angiogenesis by binding to a_v ß₃ receptors on newly formed blood vessels. These compounds may be combined with DTPA-Tyr³-octreotate and form hybrid peptides that can bind to both SSTR₂ and a_v ß₃ receptors and can thus be used for both scintigraphy and radionuclide therapy (van Hagen et al. 2000). Co-administration of chemotherapeutic drugs with radiosensitizing properties may act synergistically with irradiation, improving the efficacy of therapy with radiopharmaceuticals (Chatal et al. 2000). Immune system activation for the control of micro-metastatic disease may play an additional role in pursuit of complete control and cure. Finally, transfer of genes encoding for the expression of SSTR₂ and SSTR₅ to receptor-negative tumours may render them responsive to treatment with these agents, including radiolabelled compounds (de Herder et al. 2003, Kaltsas et al. 2004b).

	Multidisciplinary approach

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Patients with cancer and particularly patients with GEP tumours (due to their prolonged survival in the face of extensive disease) greatly benefit from a multidisciplinary therapeutic approach. Such teams should include physicians with a special interest in NETs, pathologists, endocrinologists, gastroenterologists, interventional radiologists, surgeons, oncologists and nuclear medicine experts. Diagnostic procedures should be critically reviewed to help establish a consensus on the best evidence-based management, including invasive procedures such as chemoembolization or repeated surgical procedures. The integration of receptor therapy with SST analogue (octreotide and lanreotide)-based radiopharmaceuticals and biotherapy (long-acting SST analogues with or without IFN-), together with other therapeutic approaches, must be explored. As a rule, surgical debulking of the primary tumour and reduction of hepatic deposits should be the first-line treatment; the optimal approach must be considered in each patient in order to improve survival and maintain good QoL (Kaltsas et al. 2004b). Attempts to improve QoL should be sought, as patients with metastatic disease may still experience prolonged survival, and this should be weighed against the potential side effects of any systemic treatment. Since the majority of these tumours grow slowly and even patients with disseminated disease may have prolonged survival, early involvement in palliative team programmes may be helpful. In order to evaluate the results of current management, establish guidelines and develop new therapeutic trials, multicentre collaborations are particularly helpful.

	Summary

Top Abstract Introduction Basic concepts of applying... Dosimetry Pretreatment considerations Peptide receptor radionuclide... Peptide receptor radionuclide... Peptide receptor radionuclide... Combination treatment with 90Y... Predictors of response Radioembolization Safety and side effects Future prospects Multidisciplinary approach Summary References

 
Treatment with radiolabelled SST analogues is an evolving therapeutic modality for the management of patients with inoperable or metastasized neuro-endocrine, particularly GEP, tumours. Prerequisities for treatment success include demonstration of high tumour uptake relative to non-target tissues on quantitative, diagnostic radionuclide imaging (e.g. OctreoScan) and stable haematological and biochemical function. Although there is a need for more accurate prediction of the absorbed doses, treatment with all ¹¹¹In-, ⁹⁰Y- or ¹⁷⁷Lu-labelled SS analogues has been associated with considerable symptomatic improvement with limited side effects that are mainly related to kidney function. Targeted therapies are better tolerated than other systemic treatments and deliver higher absorbed radiation doses to the tumour than can be achieved by external beam irradiation. The results obtained with [⁹⁰Y-DOTA⁰,Tyr³]-octreotide and [¹⁷⁷Lu-DOTA⁰,Tyr³]-octreotate have also exhibited substantial tumour regression, whereas the combination of these two radiopeptides may be even more effective than either alone. Future directions for improving this type of therapy include multireceptor targeting, attempts to upregulate SSTR expression on tumours and the use of radiosensitizers.

	Acknowledgements

 
The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.

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