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I Ben-Batalla, Santiago de Compostela, Spain
S Seoane, Santiago de Compostela, Spain
M Macia, Santiago de Compostela, Spain
T Garcia-Caballero, Santiago de Compostela, Spain
L Gonzalez, Gijon, Spain
F Vizoso, Gijon, Spain
R Perez-Fernandez, Physiology, University of Santiago de Compostela, Santiago de Compostela, 15782, Spain

Roman Perez-Fernandez, Email: roman.perez.fernandez{at}usc.es

Abstract

The transcription factor Pit-1/Pou1f1 regulates GH and PRL secretion in the pituitary gland. Pit-1 expression and GH regulation by Pit-1 have also been demonstrated in mammary gland. However, no data are available on the role of Pit-1 on breast PRL. To evaluate this role, several human breast cancer cell lines were transfected with either the Pit-1 expression vector or a Pit-1 siRNA construct, followed by PRL mRNA and protein evaluation. In addition, transient transfection of MCF-7 cells by a reporter construct containing the proximal PRL promoter, and ChIP assays were performed. Our data indicate that Pit-1 regulates mammary PRL at transcriptional level by binding to the proximal PRL promoter. We also found that Pit-1 raises cyclin D1 expression before increasing PRL levels, suggesting an PRL-independent effect of Pit-1 on cell proliferation. By using immunohistochemistry, we found a significant correlation between Pit-1 and PRL expression in 94 human breast invasive ductal carcinomas. Considering the possible role of PRL in breast cancer disorders, the function of Pit-1 in breast should be the focus of further research.