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This Article Accepted manuscript (PDF) All Versions of this Article: ERC-08-0030v1 15/4/965    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Laezza, C. Articles by Bifulco, M. PubMed PubMed Citation Articles by Laezza, C. Articles by Bifulco, M.

C Laezza, IEOS, CNR, Napoli, 80131, Italy
S Pisanti, Scienze Farmaceutiche, Universita  di Salerno, Salerno, Italy
A Malfitano, Scienze Farmaceutiche, Universita  di Salerno, Salerno, Italy
M Bifulco, Scienze Farmaceutiche, Universita  di Salerno, Salerno, Italy

Correspondence: Chiara Laezza, Email: chilaez{at}hotmail.com

Abstract

The endocannabinoid system regulates cell proliferation and migration in human breast cancer cells. In this study we showed that a metabolically stable analogue of anandamide, Met-F-AEA, inhibited the RhoA activity and caused a RhoA delocalization from the cell membrane to cytosol determining a decrease in actin stress fibers. Overexpression of a dominant negative of RhoA activity and treatment of these cells with a Rho-associated protein kinase (ROCK) inhibitor, Y 27632, mimicked Met-F-AEA effects on actin organization and cell migration. We suggest that the inhibitory effect of Met-F AEA on tumour cell migration could be related to RhoA-ROCK dependent signaling pathway.