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¹ Integrated Biomedical Science Graduate Program, The Ohio State University, Columbus, Ohio, USA
² The Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio, USA
³ Department of Physiology and Cell Biology, The Ohio State University, Columbus, Ohio, USA and
⁴ Department of Internal Medicine, The Ohio State University, Columbus, Ohio, USA

(Requests for offprints should be addressed to S M Jhiang who is now at 304 Hamilton Hall, 1645 Neil Avenue, The Ohio State University, Columbus, Ohio 43210, USA; Email: jhiang.1{at}osu.edu)

The Na⁺/I^– symporter (NIS)-mediated iodide uptake is the basis for targeted radioiodine ablation of thyroid cancers. However, NIS-mediated radioiodide uptake (RAIU) activity is often reduced in thyroid cancers. As mitogen activated protein kinase (MAPK) signaling pathway is activated in about 70% of papillary thyroid carcinoma, we investigated whether MEK (MAPK kinase) inhibition will restore NIS protein levels and NIS-mediated RAIU activity in RET/PTC oncogene-transformed thyroid cells. We found that MEK inhibitor PD98059 increased NIS protein levels within 30 min of treatment. However, the increase of NIS protein level was not accompanied with an increase in NIS-mediated RAIU activity, particularly at early time points of PD98059 treatment. PD98059 also decreased RAIU activity mediated by exogenous NIS in non-thyroid cells. The transient decrease of RAIU activity by PD98059 in thyroid cells was not due to decreased NIS cell surface level, decreased NIS binding affinity for I^– , or increased iodide efflux. While PD98059 moderately decreased Na⁺/K⁺-ATPase activity, ouabain titration indicates that the extent of decrease in Na⁺/K⁺-ATPase activity is much greater than the extent of decrease in RAIU activity. Additionally, a decrease of Na⁺/K⁺-ATPase activity was not accompanied with a decrease of biotin uptake activity mediated by Na⁺-dependent multivitamin transporter. Since PD98059 reduced V_max– I^– without decreasing NIS cell surface levels, it is most likely that PD98059 decreases the turnover rate of iodide transport with an yet to be identified mechanism.