ERC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Endocrine-Related Cancer 12 (2) 335 -349     DOI: 10.1677/erc.1.00898
Copyright © 2005 by the Society for Endocrinology
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lemonnier, L
Right arrow Articles by Skryma, R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lemonnier, L
Right arrow Articles by Skryma, R

Alterations in the regulatory volume decrease (RVD) and swelling-activated Cl–current associated with neuroendocrine differentiation of prostate cancer epithelial cells

L Lemonnier1,*, R Lazarenko2,*, Y Shuba2,**, S Thebault1, M Roudbaraki1, G Lepage1, N Prevarskaya1 and R Skryma1,**

1 Laboratoire de Physiologie Cellulaire, INSERM EMI 0228, USTL Bât. SN3, 59655 Villeneuve d’Ascq, France
2 Bogomoletz Institute of Physiology and International Center of Molecular Physiology, NASU, Bogomoletz Str., 4, 01024 Kiev, Ukraine

(Requests for offprints should be addressed to R Skryma; Email: phycel{at}pop.univ-lille1.fr)

Neuroendocrine (NE) differentiation of prostate epithelial/basal cells is a hallmark of advanced, androgen-independent prostate cancer, for which there is no successful therapy. Here we report for the first time on alterations in regulatory volume decrease (RVD) and its key determinant, swelling-activated Cl– current (ICl,swell), associated with NE differentiation of androgen-dependent LNCaP prostate cancer epithelial cells. NE-differentiating regimens, namely, chronic cAMP elevation or androgen deprivation, resulted in generally augmented ICl,swell and enhanced RVD. This occurred as a result of both the increased endogenous expression of ClC-3, which is a volume-sensitive Cl– channel involved, as we show, in ICl,swell in LNCaP (lymph-node carcinoma of the prostate) cells and the weaker negative ICl,swell control from Ca2+entering via store-dependent pathways. The changes in the RVD of NE-differentiated cells generally mimicked those reported for Bcl-2-conferred apoptotic resistance. Our results suggest that strengthening the mechanism that helps to maintain volume constancy may contribute to better survival rates of apoptosis-resistant NE cells.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
F. P. Thinnes
Is There Competition in Trafficking of VDAC-cored VRAC and SOC in NE Differentiation of Cells?
J. Biol. Chem., June 19, 2009; 284(25): le3 - le3.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Sato, Y. Maruyama, T. Ogura, K. Mio, K. Kato, T. Kaneko, S. Kiyonaka, and Y. Mori
Reply to Thinnes: Is There Competition in Trafficking of VDAC-cored VRAC and SOC in NE Differentiation of Cells?
J. Biol. Chem., June 19, 2009; 284(25): le4 - le4.
[Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. S. Suh, M. Mutoh, T. Mutoh, L. Li, A. Ryscavage, J. M. Crutchley, R. A. Dumont, C. Cheng, and S. H. Yuspa
CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation
J. Cell Sci., August 1, 2007; 120(15): 2631 - 2640.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the Society for Endocrinology.