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¹ Laboratoire de Physiologie Cellulaire, INSERM EMI 0228, USTL Bât. SN3, 59655 Villeneuve d’Ascq, France
² Bogomoletz Institute of Physiology and International Center of Molecular Physiology, NASU, Bogomoletz Str., 4, 01024 Kiev, Ukraine

(Requests for offprints should be addressed to R Skryma; Email: phycel{at}pop.univ-lille1.fr)

Neuroendocrine (NE) differentiation of prostate epithelial/basal cells is a hallmark of advanced, androgen-independent prostate cancer, for which there is no successful therapy. Here we report for the first time on alterations in regulatory volume decrease (RVD) and its key determinant, swelling-activated Cl^– current (I_Cl,swell), associated with NE differentiation of androgen-dependent LNCaP prostate cancer epithelial cells. NE-differentiating regimens, namely, chronic cAMP elevation or androgen deprivation, resulted in generally augmented I_Cl,swell and enhanced RVD. This occurred as a result of both the increased endogenous expression of ClC-3, which is a volume-sensitive Cl^– channel involved, as we show, in I_Cl,swell in LNCaP (lymph-node carcinoma of the prostate) cells and the weaker negative I_Cl,swell control from Ca²⁺entering via store-dependent pathways. The changes in the RVD of NE-differentiated cells generally mimicked those reported for Bcl-2-conferred apoptotic resistance. Our results suggest that strengthening the mechanism that helps to maintain volume constancy may contribute to better survival rates of apoptosis-resistant NE cells.

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